重组DNA
计算生物学
遗传增强
效价
载体(分子生物学)
体内
基因传递
计算机科学
生化工程
生物
病毒载体
化学
病毒学
基因
生物技术
病毒
遗传学
工程类
作者
Lyubava Belova,Konstantin S. Kochergin-Nikitsky,Anastasia Erofeeva,А. В. Лавров,Svetlana Smirnikhina
出处
期刊:BioEssays
[Wiley]
日期:2022-04-14
卷期号:44 (6)
被引量:2
标识
DOI:10.1002/bies.202200019
摘要
Recombinant adeno-associated viruses (rAAVs) are promising vectors for the delivery of various genetic constructs into eukaryotic cells. rAAVs have a number of properties that make it possible to successfully use them both in vitro and in vivo. Purification and concentration of rAAV vectors are critical for achieving high viral titer, stability, efficiency, and purity. This review systematically analyses all available purification approaches. The purification methods described in this work differ substantially from each other in mechanisms, efficiency, labor time, and cost. Researchers have to choose a purification algorithm depending on the purpose of their work. We strive to simplify the choice of the necessary and sufficient technique based on the experimental needs and available resources of the laboratory.
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