再生(生物学)
组织工程
生物医学工程
脚手架
间充质干细胞
3D生物打印
作者
Zheng Zhong,Alis Balayan,Jing Tian,Yi Xiang,Hanjun Henry Hwang,Xiaokang Wu,Xiaoqian Deng,Jacob Schimelman,Yazhi Sun,Chao Ma,Aurélie Dos Santos,Shangting You,Min Tang,Emmie Yao,Xiaoao Shi,Nicole F. Steinmetz,Sophie X. Deng,Shaochen Chen
出处
期刊:Biofabrication
[IOP Publishing]
日期:2021-08-13
卷期号:13 (4): 044101-
标识
DOI:10.1088/1758-5090/ac1992
摘要
Limbal stem cell deficiency (LSCD) and corneal disorders are among the top global threats for human vision. Emerging therapies that integrate stem cell transplantation with engineered hydrogel scaffolds for biological and mechanical support are becoming a rising trend in the field. However, methods for high-throughput fabrication of hydrogel scaffolds, as well as knowledge of the interaction between limbal stem/progenitor cells (LSCs) and the surrounding extracellular matrix (ECM) are still much needed. Here, we employed digital light processing (DLP)-based bioprinting to fabricate hydrogel scaffolds encapsulating primary LSCs and studied the ECM-dependent LSC phenotypes. The DLP-based bioprinting with gelatin methacrylate (GelMA) or hyaluronic acid glycidyl methacrylate (HAGM) generated microscale hydrogel scaffolds that could support the viability of the encapsulated primary rabbit LSCs (rbLSCs) in culture. Immunocytochemistry and transcriptional analysis showed that the encapsulated rbLSCs remained active in GelMA-based scaffolds while exhibited quiescence in the HAGM-based scaffolds. The primary human LSCs (hLSCs) encapsulated within bioprinted scaffolds showed consistent ECM-dependent active/quiescent statuses. Based on these results, we have developed a novel bioprinted dual ECM 'Yin-Yang' model encapsulating LSCs to support both active and quiescent statues. Our findings provide valuable insights towards stem cell therapies and regenerative medicine for corneal reconstruction.
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