化学
DNA
拉曼光谱
DNA–DNA杂交
杂交探针
表面增强拉曼光谱
DNA测序
核酸热力学
序列(生物学)
生物物理学
生物化学
基序列
拉曼散射
光学
生物
物理
作者
Aoune Barhoumi,Naomi J. Halas
摘要
The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm(-1); the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.
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