效应器
黄单胞菌
生物
背景(考古学)
核苷酸
计算生物学
基因
蛋白质-DNA相互作用
遗传学
DNA
机制(生物学)
DNA结合蛋白
转录因子
细胞生物学
认识论
哲学
古生物学
作者
Matthew J. Moscou,Adam J. Bogdanove
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2009-12-11
卷期号:326 (5959): 1501-1501
被引量:1712
标识
DOI:10.1126/science.1178817
摘要
TAL effectors of plant pathogenic bacteria in the genus Xanthomonas bind host DNA and activate genes that contribute to disease or turn on defense. Target specificity depends on an effector-variable number of typically 34 amino acid repeats, but the mechanism of recognition is not understood. We show that a repeat-variable pair of residues specifies the nucleotides in the target site, one pair to one nucleotide, with no apparent context dependence. Our finding represents a previously unknown mechanism for protein-DNA recognition that explains TAL effector specificity, enables target site prediction, and opens prospects for use of TAL effectors in research and biotechnology.
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