Demethylation of the SFRP4 Promoter Drives Gastric Cancer Progression via the Wnt Pathway

Wnt信号通路 癌症研究 癌症 肿瘤进展 癌变 甲基化 生物 转移 干瘪的 癌细胞 大肠腺瘤性息肉病 表观遗传学 去甲基化 癌症干细胞 DNA甲基化 干细胞 脱甲基酶 信号转导 基因表达 细胞生物学 基因 遗传学
作者
Haojie Li,Junjie Zhao,Jie Sun,Chuanshan Tian,Quan Jiang,Chen Ding,Qiangjun Gan,Ping Shu,Xuefei Wang,Jing Qin,Yihong Sun
出处
期刊:Molecular Cancer Research [American Association for Cancer Research]
卷期号:19 (9): 1454-1464 被引量:6
标识
DOI:10.1158/1541-7786.mcr-20-0933
摘要

Abstract Wnt signaling is believed to be an important contributor to tumor development and has been reported to be modulated by secreted frizzled-related proteins (SFRP). Nevertheless, the role of secreted frizzled-related protein 4 (SFRP4) in tumorigenesis remains controversial. We aim to explore its biological function in gastric cancer. Genomes analysis based on the Gene Expression Omnibus (GEO) dataset was used to find the differential gene expression between different tumor–node–metastasis (TNM) stages of gastric cancer. IHC was used to determine the relationship between SFRP4 expression and clinicopathologic characteristics in patients with gastric cancer. The influence of SFRP4 on tumor progression was evaluated by CCK-8, colony formation, cell apoptosis, and cell cycle in vitro, as well as xenograft model in vivo. The methylation status of SFRPs was examined in gastric cancer specimens by quantitative methylation analysis. SFRP4 was most upregulated in advanced gastric cancer. High intratumoral SFRP4 expression, which was associated with tumor invasion and metastasis, was also a poor prognostic indicator for patients with gastric cancer. In vitro and in vivo studies revealed that SFRP4 could promote tumor growth; however, IWR-1 could suppress tumor growth mediated by SFRP4 overexpression. Mechanistic exploration found that SFRP4 was overexpressed by the decrease of promoter methylation and thus could competitively antagonize the inhibitory effect of SFRP1 on Wnt pathway activation and tumor progression in gastric cancer. Implications: In gastric cancer, the expression of SFRP4 was upregulated by decreased methylation. High intratumoral SFRP4 expression could activate the Wnt pathway to promote tumor progression and predict poor survival of patients with gastric cancer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
左一发布了新的文献求助10
刚刚
雨洋完成签到,获得积分10
刚刚
SciGPT应助风中傻姑采纳,获得10
刚刚
SmallYu完成签到,获得积分10
1秒前
4秒前
舒适思烟发布了新的文献求助10
5秒前
一宿发布了新的文献求助50
7秒前
车依庭发布了新的文献求助10
8秒前
求助完成签到,获得积分10
9秒前
9秒前
小蒋完成签到 ,获得积分10
10秒前
12秒前
fuyishuai发布了新的文献求助10
15秒前
liu完成签到,获得积分10
17秒前
19秒前
腾飞完成签到 ,获得积分10
20秒前
上官若男应助奋斗嫣然采纳,获得30
21秒前
学垃圾的小物理完成签到,获得积分20
21秒前
风中傻姑发布了新的文献求助10
22秒前
perry4rosa应助猩心采纳,获得10
25秒前
天天快乐应助科研巨额采纳,获得10
27秒前
之_ZH完成签到 ,获得积分10
29秒前
29秒前
一宿发布了新的文献求助50
30秒前
herdwind完成签到,获得积分10
31秒前
31秒前
themanell完成签到,获得积分10
33秒前
orixero应助科研通管家采纳,获得10
36秒前
在水一方应助科研通管家采纳,获得10
36秒前
完美世界应助科研通管家采纳,获得10
36秒前
benben应助科研通管家采纳,获得10
36秒前
aldehyde应助科研通管家采纳,获得10
36秒前
科研通AI2S应助科研通管家采纳,获得20
36秒前
aldehyde应助科研通管家采纳,获得10
36秒前
cc完成签到,获得积分10
37秒前
杨刚发布了新的文献求助10
37秒前
37秒前
打可爱完成签到,获得积分10
37秒前
JamesPei应助Angie格琳采纳,获得10
39秒前
完美世界应助huyang采纳,获得10
40秒前
高分求助中
Sraffa and applied economics: are there classical supply curves? 1000
Heun’s Differential Equations 600
《Fundamentals of Power Supply Design》Robert A. Mammano 510
谁能看见前面有梦可想 500
Wind Effects on Structures: An Introduction to Wind Engineering and Wind Forces in Engineering (2nd Edition) 300
Against the Grain: The Vayda Tradition in Human Ecology and Ecological Anthropology 300
High Wire: How China Regulates Big Tech and Governs Its Economy 260
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2248442
求助须知:如何正确求助?哪些是违规求助? 1897815
关于积分的说明 4731602
捐赠科研通 1686927
什么是DOI,文献DOI怎么找? 855929
版权声明 545005
科研通“疑难数据库(出版商)”最低求助积分说明 453031