3D生物打印
翼状胬肉
血管生成
新生血管
转录组
医学
癌症研究
生物
组织工程
生物医学工程
眼科
生物化学
基因
基因表达
作者
Zheng Zhong,Jonathan T. Wang,Jing Tian,Xiaoqian Deng,Alis Balayan,Yazhi Sun,Yi Xiang,Jiaao Guan,Jacob Schimelman,Henry Hwang,Shangting You,Xiaokang Wu,Chao Ma,Xiaoao Shi,Emmie Yao,Sophie X Deng,Shaochen Chen
出处
期刊:Biomaterials
[Elsevier]
日期:2022-03-01
卷期号:282: 121391-121391
被引量:3
标识
DOI:10.1016/j.biomaterials.2022.121391
摘要
Pterygium is an ocular surface disorder with high prevalence that can lead to vision impairment. As a pathological outgrowth of conjunctiva, pterygium involves neovascularization and chronic inflammation. Here, we developed a 3D multicellular in vitro pterygium model using a digital light processing (DLP)-based 3D bioprinting platform with human conjunctival stem cells (hCjSCs). A novel feeder-free culture system was adopted and efficiently expanded the primary hCjSCs with homogeneity, stemness and differentiation potency. The DLP-based 3D bioprinting method was able to fabricate hydrogel scaffolds that support the viability and biological integrity of the encapsulated hCjSCs. The bioprinted 3D pterygium model consisted of hCjSCs, immune cells, and vascular cells to recapitulate the disease microenvironment. Transcriptomic analysis using RNA sequencing (RNA-seq) identified a distinct profile correlated to inflammation response, angiogenesis, and epithelial mesenchymal transition in the bioprinted 3D pterygium model. In addition, the pterygium signatures and disease relevance of the bioprinted model were validated with the public RNA-seq data from patient-derived pterygium tissues. By integrating the stem cell technology with 3D bioprinting, this is the first reported 3D in vitro disease model for pterygium that can be utilized for future studies towards personalized medicine and drug screening.
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