体内
膜
药物输送
癌细胞
转移
癌症研究
循环肿瘤细胞
材料科学
体外
细胞粘附
癌症
细胞
化学
生物物理学
细胞生物学
纳米技术
生物
生物化学
遗传学
生物技术
作者
Ting Kang,Qianqian Zhu,Dan Wei,Jingxian Feng,Jianhui Yao,Tianze Jiang,Qing Song,Xunbin Wei,Hongzhuan Chen,Xiaoling Gao,Jun Chen
出处
期刊:ACS Nano
[American Chemical Society]
日期:2017-01-26
卷期号:11 (2): 1397-1411
被引量:387
标识
DOI:10.1021/acsnano.6b06477
摘要
The dissemination, seeding, and colonization of circulating tumor cells (CTCs) serve as the root of distant metastasis. As a key step in the early stage of metastasis formation, colonization of CTCs in the (pre-)metastatic niche appears to be a valuable target. Evidence showed that inflammatory neutrophils possess both a CTC- and niche-targeting property by the intrinsic cell adhesion molecules on neutrophils. Inspired by this mechanism, we developed a nanosize neutrophil-mimicking drug delivery system (NM-NP) by coating neutrophils membranes on the surface of poly(latic-co-glycolic acid) nanoparticles (NPs). The membrane-associated protein cocktails on neutrophils membrane were mostly translocated to the surface of NM-NP via a nondisruptive approach, and the biobinding activity of neutrophils was highly preserved. Compared with uncoated NP, NM-NP exhibited enhanced cellular association in 4T1 cell models under shear flow in vitro, much higher CTC-capture efficiency in vivo, and improved homing to the premetastatic niche. Following loading with carfilzomib, a second generation of proteasome inhibitor, the NM-NP-based nanoformulation (NM-NP-CFZ) selectively depleted CTCs in the blood, prevented early metastasis and potentially inhibited the progress of already-formed metastasis. Our NP design can neutralize CTCs in the circulation and inhibit the formation of a metastatic niche.
科研通智能强力驱动
Strongly Powered by AbleSci AI