Non-viral precision T cell receptor replacement for personalized cell therapy

T细胞受体 嵌合抗原受体 T细胞 生物 人类白细胞抗原 转基因 基因组编辑 免疫疗法 癌症研究 清脆的 免疫学 基因 免疫系统 抗原 遗传学
作者
Susan P. Foy,Kyle Jacoby,Daniela A. Bota,Theresa Hunter,Zheng Pan,Eric Stawiski,Yan Ma,William Lu,Songming Peng,Clifford L Wang,Benjamin T. K. Yuen,Olivier Dalmas,Katharine Heeringa,Barbara Sennino,Andy Conroy,Michael T. Bethune,Ines Mende,William White,Monica Kukreja,Swetha Gunturu,Emily Humphrey,Adeel Hussaini,Duo An,Adam J. Litterman,Boi Quach,Alphonsus H. C. Ng,Yue Lu,Chad Smith,Katie M. Campbell,Daniel Anaya,Lindsey Skrdlant,Eva Yi-Hsuan Huang,Ventura Mendoza,Jyoti Mathur,Luke Dengler,Bhamini Purandare,Robert Moot,Michael S. Yi,Roel Funke,Alison Sibley,Todd Stallings-Schmitt,David Y Oh,Bartosz Chmielowski,Mehrdad Abedi,Yuan Yuan,Jeffrey A Sosman,Sylvia M Lee,Adam J Schoenfeld,David Baltimore,James R. Heath,Alex Franzusoff,Antoni Ribas,Arati Rao,Stefanie Mandl
出处
期刊:Nature [Springer Nature]
卷期号:615 (7953): 687-696 被引量:27
标识
DOI:10.1038/s41586-022-05531-1
摘要

T cell receptors (TCRs) enable T cells to specifically recognize mutations in cancer cells1-3. Here we developed a clinical-grade approach based on CRISPR-Cas9 non-viral precision genome-editing to simultaneously knockout the two endogenous TCR genes TRAC (which encodes TCRα) and TRBC (which encodes TCRβ). We also inserted into the TRAC locus two chains of a neoantigen-specific TCR (neoTCR) isolated from circulating T cells of patients. The neoTCRs were isolated using a personalized library of soluble predicted neoantigen-HLA capture reagents. Sixteen patients with different refractory solid cancers received up to three distinct neoTCR transgenic cell products. Each product expressed a patient-specific neoTCR and was administered in a cell-dose-escalation, first-in-human phase I clinical trial ( NCT03970382 ). One patient had grade 1 cytokine release syndrome and one patient had grade 3 encephalitis. All participants had the expected side effects from the lymphodepleting chemotherapy. Five patients had stable disease and the other eleven had disease progression as the best response on the therapy. neoTCR transgenic T cells were detected in tumour biopsy samples after infusion at frequencies higher than the native TCRs before infusion. This study demonstrates the feasibility of isolating and cloning multiple TCRs that recognize mutational neoantigens. Moreover, simultaneous knockout of the endogenous TCR and knock-in of neoTCRs using single-step, non-viral precision genome-editing are achieved. The manufacture of neoTCR engineered T cells at clinical grade, the safety of infusing up to three gene-edited neoTCR T cell products and the ability of the transgenic T cells to traffic to the tumours of patients are also demonstrated.
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