拉曼光谱
表面增强拉曼光谱
肽核酸
检出限
DNA
拉曼散射
基质(水族馆)
胶体金
对苯二酚
核酸
材料科学
银纳米粒子
化学
分析化学(期刊)
共振拉曼光谱
光谱学
光化学
罗丹明6G
纳米颗粒
组合化学
纳米技术
色谱法
生物化学
光学
生物
物理
生态学
作者
Yong Qian,Taotao Fan,Yao Yao,Xin Shi,Xianjiu Liao,Fuyi Zhou,Fenglei Gao
标识
DOI:10.1016/j.snb.2017.07.112
摘要
Abstract In this work, a label-free and Raman dyes-free surface enhanced Raman spectroscopy for DNA detection based on the in situ DNA-metallization was developed and studied. A glass slide was employed to immobilize a peptide nucleic acid (PNA) as a recognition probe and to perform the detection procedure. Upon hybridization of the target DNA with the PNA probe, the DNA skeleton could adsorb positively charged silver ions due to the negatively charged DNA target. After chemical reduction by hydroquinone followed by a silver enhancement step, silver nanoparticles could be grown on the surface to reach 10 nm in size. The grown silver nanoparticles along the DNA skeleton induced sufficient interactions between the bases and the substrate to yield a sensitive Raman signal. The results suggested that the SERS spectrum of DNA was strongly dominated by the spectral feature of adenine at 736 cm−1, where adenine served as an endogenous marker for label-free SERS detection of DNA. Using this method, highly reproducible and good quality SERS signals of DNA were obtained with a linear range varied between 1.0 × 10−10 to 1.0 × 10−6 M and detection limit of 34 pM. Overall, this high-performance DNA-metallization based SERS method may offer a versatile platform for label-free SERS detection of DNA at low-cost with promising application in clinical diagnosis.
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