清脆的
计算生物学
基因组编辑
生物
基因组
广谱
遗传学
核酸
基因
化学
组合化学
作者
Kyle E. Watters,Christof Fellmann,Hua B. Bai,Shawn M. Ren,Jennifer A. Doudna
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2018-10-12
卷期号:362 (6411): 236-239
被引量:174
标识
DOI:10.1126/science.aau5138
摘要
Cas12 inhibitors join the anti-CRISPR family Bacteria and their phages continually coevolve in a molecular arms race. For example, phages use anti-CRISPR proteins to inhibit the bacterial type I and II CRISPR systems (see the Perspective by Koonin and Makarova). Watters et al. and Marino et al. used bioinformatic and experimental approaches to identify inhibitors of type V CRISPR-Cas12a. Cas12a has been successfully engineered for gene editing and nucleic acid detection. Some of the anti-Cas12a proteins identified in these studies had broad-spectrum inhibitory effects on Cas12a orthologs and could block Cas12a-mediated genome editing in human cells. Science , this issue p. 236 , p. 240 ; see also p. 156
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