色谱法
化学
氢氧化钠
季枯
氢氧化铵
基质(化学分析)
固相萃取
样品制备
串联质谱法
萃取(化学)
质谱法
百草枯
生物化学
有机化学
物理化学
作者
Emad R. Attallah,Mostafa Soliman,Mohamed M. Abo-Aly
标识
DOI:10.5740/jaoacint.16-0315
摘要
A simple, modified quick, easy, cheap, effective, rugged, and safe procedure was developed for the determination of diquat in potato using reversed-phase LC coupled with tandem MS (MS/MS) in a total run time of 10 min. Different sample preparation parameters (pH modifier type, sample size, and elevated temperature) were tested and optimized. Potato sample was extracted with acetonitrile in the presence of ammonium hydroxide at 80°C. Phase separation was obtained by shaking the extract with magnesium sulfate and sodium chloride, and analysis was done using LC-MS/MS. Matrix-matched standard calculations were used to compensate for matrix-induced suppression in LC-MS/MS determination. The precision and trueness of the method were determined from recovery experiments on five replicates of spiked blank potato samples at 0.01, 0.05, and 0.1 mg/kg. The range of the obtained recoveries was 74-110%, with RSD values <5% for all the concentrations.
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