Detection of replication sites in plant cell nuclei by using semithin sections

A novel approach for detection of replication sites in plant cells nuclei is described. The included nucleotide (EdU) was revealed by using “click” chemistry in semithin sections of the material embedded in acrylic resin. The use of the proposed protocol allows (1) to achieve good preservation of cell morphology, (2) to work with any tissues, and (3) to obtain high resolution during microscopy (especially, axial).