Mouse meninges isolation for FACS

Presented is a method for removal of meninges from the brain and interior skull of the mouse yielding tissue suitable for preparing a single-cell suspension amenable to downstream applications such as ow cytometric analysis or short-term cell culture. ReagentsNembutal similar, as approved by your governing body) Perfusion apparatus suitable for transcardial mouse perfusion Perfusion Buffer \(0.1MPBS/5u/ml sodium heparin) FACS Buffer \(0.1M PBS \(pH 7.4)/2mM EDTA/1% BSA)