Portable organophosphorus pesticide detection device based on controllable microfluidic and luminol composite nanofibers

Excessive organophosphorus pesticide residues in food are a serious threat to human health, it is very urgent to develop a portable, low-cost, and high precision pesticide residue detection device to achieve rapid detection of organophosphorus pesticide residues. In this study, a portable organophosphorus pesticide detection device equipped with microfluidic and luminol composite nanofibers was developed. The microfluidic chip was designed and optimized by a combine of mathematical model and multi-physics coupling simulation. The luminol particles were solidly loaded on polyacrylonitrile (PAN) electrospinning film through electrospinning method to achieve efficient utilization of microfluidic chips and luminol fluorescent reagents. The influence of the mass fraction of luminol particle on the microstructure of electrospinning film was analyzed, and the optimal mass fraction was determined by measuring the fluorescent stability and fluorescent intensity. The electrospinning film and the microfluidic chip was loaded to an independently developed portable organophosphorus pesticide detection device, whose detection limit can reach 0.035 mg/L and the average detection time can reach 30 s. The portable organophosphorus pesticide detection device shows high stability, high detection accuracy and efficiency, which can be widely adopted in the detection of organophosphorus pesticides.