Antioxidant and protective effects of black Punica granatum extract on AGEs and UV‐A‐induced damaged human fibroblast (647.28)

One of causes of skin wrinkles may be the nonenzymatic glycation followed formation of browning products called advanced glycation endproducts (AGEs), an irreversible cross‐linked protein. The accumulation of glycated collagen cross‐linked in skin inhibits the formation and function of skin tightening agents such as collagen. Also, the UV effects on AGEs generates reactive oxygen species (ROS) in the extracellular matrix which could lead to additional deleterious effects on skin. Antioxidant, glycation inhibitory activities and anti‐skin aging of Punica granatum were have been founded and it is known that ellagic acid (EA), major compound of Punica granatum , has antioxidant effect. This study analyzed the functional components, antioxidant activities and anti‐skin aging of black Punica granatum extracts (BPE). Previous study reported that significant correlations were found between the red intensity, antioxidant capacity and total phenolic content. And we confirmed that BPE had more antioxidant activity, total polyphenol, total flavonoid and ellagic acid content than Punica granatum . Moreover, we studied photoprotective effect of BPE that attenuated the AGEs and UV‐A‐induced toxicity of human fibroblast. Also, this study examined potent activities of BPE on type 1 collagen synthesis and elastase inhibitory activity. In conclusion, we demonstrated the beneficial effects of black Punica granatum on anti‐wrinkle and the improving effect on damaged skin induced by AGEs and UV‐A.