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亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!
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2025-04-19 加入
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Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth disease virus
1个月前
已完结
First isolation and whole-genome characterization of a G9P[14] rotavirus strain from a diarrheic child in Egypt
1个月前
已完结
Isolation and characterization of a rare group A rotavirus G13P[18] strain from a diarrhoeic foal in Japan
1个月前
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A provisional complete genome-based genotyping system for rotavirus species C from terrestrial mammals
1个月前
已完结
Refining genetic classification of global porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and investigating their geographic and temporal distributions
4个月前
已完结
PUS1 facilitates cell migration in clear cell renal cell carcinoma through the promotion of mRNA pseudouridylation and the stabilization of the SMOX gene
1个月前
已采纳
First-principles investigation of the physical characteristic of ternary rare-earth superconductor LuPd2Si2
1个月前
已采纳
Study of morphological, elemental, optical and excitation wavelength dependent red photoluminescence in Eu3+ doped Li2SrSiO4 for solid state lighting
1个月前
已采纳
Visible LED active photocatalyst based on cerium doped titania for Rhodamine B degradation: Radical's contribution, stability and response surface methodology optimization
1个月前
已采纳
Gd and Ni co-doped BiFeO3 ferrite/r-GO nanocomposite for photocatalytic environmental remediation applications
1个月前
已采纳
Effect of copper introduction on the properties of micro-arc oxidation coating on powder metallurgy aluminum disk
1个月前
已采纳
Dysprosium doped SrCeO3 nanophosphors for advanced photochemical applications: Synthesis, characterization, photo-assisted dye degradation and latent fingerprint visualization
1个月前
已采纳
An efficient approach to develop and screen out high-entropy alloy composition with high performance for biomedical application
1个月前
已采纳
TiO2 Nanotubes Array Based Ti/TiO2NTs/SnO2-Sb-Bi/NATO Electrode for Electrosynthesis of Ozone Water and Application in Wastewater Treatment
1个月前
已采纳
Jeff=12 Mott-insulating to S = 0 metal transition in Ce@Y-doped Y
1个月前
已采纳
第二页结尾处内容是 Design of primer amplification of porcine IgG variable genes Nested PCR primers were designed for amplification of variable genes of porcine gamma, kappa and lambda chains. For gamma and lambda chains, the outer and inner forward primers were both designed in the signal peptide and FR1 regions, respectively. For the kappa chain, the outer and 第三页左手边出现空白,此段内容缺陷 紧接着是新的内容: Single-cell PCR amplification and cloning of variable region genes of IgG Variable region genes of antibodies were amplified using a nested PCR method with primers for porcine IgG (Table 1). The first PCR was set up in a 25 µl reaction mixture containing 3 µl cDNA using HotStar Taq Master Mix (Qiagen), and the PCR parameters were as follows:
1个月前
第2页到第3页,衔接第2页的左手边有一部分内容缺陷而空白
1个月前
速度真快,帮大忙了
1个月前
感谢,帮大忙了,速度真快
1个月前
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