多囊肾病
肾
环磷酸腺苷
常染色体显性多囊肾病
囊肿
病理
细胞生物学
生物
福斯科林
体外
内分泌学
内科学
医学
生物化学
受体
作者
Sayanthooran Saravanabavan,Gopala K. Rangan
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 135-144
标识
DOI:10.1007/978-1-0716-3179-9_10
摘要
The formation and growth of kidney cysts (fluid-filled structures lined by epithelial cells) is the primary pathological abnormality in polycystic kidney disease (PKD). Multiple molecular pathways are disrupted in kidney epithelial precursor cells, which lead to altered planar cell polarity, increased proliferation, and fluid secretion, which together with extracellular matrix remodelling culminates in the formation and growth of cysts. Three-dimensional (3D) in vitro cyst models serve as suitable preclinical models to screen candidate drugs for PKD. Madin-Darby Canine Kidney (MDCK) epithelial cells form polarized monolayers with a fluid-filled lumen when suspended in a collagen gel, and their growth is accelerated with the addition of forskolin, a cyclic adenosine monophosphate (cAMP) agonist. Candidate drugs for PKD can be screened for their ability to modulate growth of forskolin-treated MDCK cysts by measuring and quantifying cyst images acquired at progressive timepoints. In this chapter, we describe the detailed methods for the culture and growth of MDCK cysts in a collagen matrix and a protocol for their use in testing candidate drugs to prevent cyst formation and growth.
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