| 标题 |
 Quantitative analyses of nitrogen cycling genes in soils 土壤氮循环基因的定量分析
相关领域
自行车
固氮
氮气循环
土壤水分
生物
氮气
丰度（生态学）
基因
相对物种丰度
微生物生态学
植物
环境化学
生态学
化学
遗传学
细菌
历史
考古
有机化学
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| 网址 | |
| DOI |
10.1016/i.pedobi.2005.05.005
doi
提醒：求助人提供的doi与AI识别不一致
10.1016/j.pedobi.2005.05.005
Doi
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| 其它 |
期刊：Pedobiologia [Elsevier] 日期：2005-08-18 卷期号：49 (6): 665-672 被引量：113 DOI：10.1016/j.pedobi.2005.05.005 Soil nitrogen cycling is accomplished by a diverse microbial community using several nitrogen functional genes (NFGs). The relationship between the composition of these communities and nitrogen cycling processes is poorly understood. We developed techniques to quantify several important NFGs nitrogen transformations including fixation (nifH), ammonia oxidation (amoA), nitrogen reduction (nirS and nirK), and nitrous oxide reduction (nosZ) using quantitative real-time PCR with SybrGreen. Standard curves with known gene copies of each target molecule were developed using genomic DNA extracted from pure cultures. Primers from previously published studies were successfully used to amplify and quantify each NFGs in DNA extracted from two forest soils. Some soil samples inhibited PCR amplification of positive control DNA, |
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