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Small-Scale Protein Production With the Baculovirus Expression Vector System
杆状病毒表达载体系统的小规模蛋白质生产
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其它 | Improved methods of baculovirus cloning and insect cell culture and their commercialization have made the use of the baculovirus expression vector system (BEVS) a routine tool for the production of preparative quantities of recombinant protein. This chapter outlines basic techniques for small-scale protein production using the BEVS, including protocols for expression from adherent and suspension insect cell cultures, titer estimation, and expression optimization. |
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