| 标题 |
 The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity 外显子连接核心复合物通过抑制eIF4AIII ATP酶活性锁定在RNA上
相关领域
细胞生物学
核糖核酸
RNA结合蛋白
RNA剪接
剪接体
生物
多蛋白复合物
重组DNA
化学
分子生物学
遗传学
基因
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| 其它 | The multiprotein exon junction complex (EJC) is assembled on mRNAs as a consequence of splicing. EJC core components maintain a stable grip on mRNAs even as the overall EJC protein composition evolves while mRNAs travel to the cytoplasm. Here we show that recombinant EJC subunits MLN51, MAGOH and Y14, together with the DEAD-box protein eIF4AIII bound to ATP, are necessary and sufficient to form a highly stable complex on single-stranded RNA. Cross-linking and RNase protection studies indicate that this recombinant complex recapitulates the EJC core. The stable association of the recombinant EJC core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. We elucidate the modalities of EJC binding to RNA and provide the first example of how cellular machineries may use RNA helicases to clamp several proteins onto RNA in stable and sequence-independent manners. |
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