自噬
衰老
软骨细胞
基因敲除
软骨
骨关节炎
小干扰RNA
细胞生物学
细胞
生物
医学
癌症研究
病理
细胞培养
转染
解剖
细胞凋亡
生物化学
遗传学
替代医学
作者
Yanqing Gu,Yan Ren,Yang Wang,Yiwen Zeng,Qingqiang Yao
出处
期刊:Aging
[Impact Journals, LLC]
日期:2022-07-01
卷期号:14 (13): 5366-5375
标识
DOI:10.18632/aging.204066
摘要
Objective: Osteoarthritis is closely related to aging. Tribbles homologue 3 (TRB3) is found to display age-related expression and contributes to the regulation of cell proliferation, differentiation and fibrosis. In this study, we aimed to investigate the potential involvement of TRB3 in cartilage autophagy and aging in osteoarthritis. Methods: Cartilage tissue samples were collected from osteoarthritis patients who received joint replacement and cadaveric donors. In osteoarthritis cartilage tissue, we analyzed autophagy- and senescence-associated proteins using immunohistochemistry and western blot (WB), in vitro, to confirm the role played by TRB3 in the process of autophagy, cell senescence, and inflammation, small interfering RNA (siRNA) was used for TRB3 knockdown in cells. Results: We found increased level of p62, decreased level of microtubule-associated protein 1A/1B-light chain 3 (LC3) and beclin-1 in cartilage, and increased level of p16 and p21 in tissue samples collected from osteoarthritis patients, indicating decreased autophagy and increased cell senescence. TRB3 knockdown significantly rescued, in vitro, the reduced autophagy and elevated cell senescence in human chondrocyte. Conclusions: Interfering with TRB3 expression in cartilage may serve as a target in the prevention and treatment of age-related osteoarthritis.
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