费斯特共振能量转移
化学
生物传感器
色谱法
适体
免疫分析
检出限
荧光
生物化学
分子生物学
生物
抗体
量子力学
物理
免疫学
作者
Hui-Jun Fu,Ruifang Su,Lin Luo,Zijian Chen,Thomas Just Sørensen,Niko Hildebrandt,Zhen-Lin Xu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-03-21
卷期号:7 (4): 1113-1121
被引量:8
标识
DOI:10.1021/acssensors.2c00085
摘要
Histamine (HA) is an indicator of food freshness and quality. However, high concentrations of HA can cause food poisoning. Simple, rapid, sensitive, and specific quantification can enable efficient screening of HA in food and beverages. However, conventional assays are complicated and time-consuming, as they require multiple incubation, washing, and separation steps. Here, we demonstrate that time-gated Förster resonance energy transfer (TG-FRET) between terbium (Tb) complexes and organic dyes can be implemented in both immunosensors and aptasensors for simple HA quantification using a rapid, single-step, mix-and-measure assay format. Both biosensors could quantify HA at concentrations relevant in food poisoning with limits of detection of 0.19 μg/mL and 0.03 μg/mL, respectively. Excellent specificity was documented against the structurally similar food components tryptamine and l-histidine. Direct applicability of the TG-FRET assays was demonstrated by quantifying HA in spiked fish and wine samples with both excellent concentration recovery and agreement with conventional multistep enzyme-linked immunosorbent assays (ELISAs). Our results show that the simplicity and rapidity of TG-FRET assays do not compromise sensitivity, specificity, and reliability, and both immunosensors and aptasensors have a strong potential for their implementation in advanced food safety screening.
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