PLGA公司
化学
聚合物
圆二色性
单体
色谱法
十二烷基硫酸钠
化学工程
牛血清白蛋白
核化学
高分子化学
有机化学
生物化学
工程类
体外
作者
Chenhui Wang,Changhui Yu,Kongtong Yu,Lesheng Teng,Jiaxin Liu,Xuesong Wang,Fengying Sun,Youxin Li
出处
期刊:Current Drug Delivery
[Bentham Science]
日期:2015-12-28
卷期号:12 (6): 726-735
被引量:2
标识
DOI:10.2174/1567201812666150316112635
摘要
The use of biodegradable polymers such as PLGA to encapsulate therapeutic proteins for their controlled release has received tremendous interest. However, an acidic environment caused by PLGA degradation productions leads to protein incomplete release and chemical degradation. The aim of this study was to develop novel PCADK/PLGA microspheres to improve protein stability and release behavior. Bovine serum albumin (BSA) incubated in PCADK and PLGA degradation products was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), size exclusion chromatography (SEC-HPLC), circular dichroism (CD) and fluorescence spectroscopy. Blended microspheres of PCADK/PLGA were prepared in different ratios and the release behaviors of the microspheres and the protein stability were then measured. The degradation properties of the microspheres and the pH inside the microspheres were systematically investigated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) to examine the mechanism of autocatalytic degradation and protein stability. BSA was more stable in the presence of PCADK monomers than it was in the presence of PLGA monomers, revealing that PCADK is highly compatible with this protein. PCADK/PLGA microspheres were successfully prepared, and 2/8 was determined to be the optimal ratio. Further, 43% of the BSA formed water-insoluble aggregates in the presence of PCADK/PLGA microspheres, compared with 57% for the PLGA microspheres, demonstrating that the BSA encapsulated in PCADK/PLGA blended microspheres was more stable than in PLGA microspheres. The PCADK/PLGA blended microspheres improved protein stability and release behavior, providing a promising protein drug delivery system. Keywords: Biodegradable polymers, microspheres, PCADK, pH, PLGA, protein drug.
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