磷酸化
内化
内吞作用
激酶
内体
互补
双分子荧光互补
细胞生物学
化学
免疫沉淀
丝氨酸
拟南芥
蛋白质片段互补分析
生物化学
生物
受体
基因
表型
突变体
作者
Thibault Kosuth,Alexandra Lešková,Reyes Ródenas,Grégory Vert,Catherine Curie,Loren Castaings
出处
期刊:FEBS Letters
[Wiley]
日期:2023-08-01
卷期号:597 (16): 2048-2058
标识
DOI:10.1002/1873-3468.14706
摘要
Manganese (Mn) is essential for plants but is toxic when taken up in excess. To maintain Mn homeostasis, the root Mn transporter natural resistance associated macrophage protein 1 (NRAMP1) cycles from the plasma membrane to endosomes upon phosphorylation. To identify the kinase involved, a split-luciferase screening was carried out between NRAMP1 and kinases of the CIPK family and identified CIPK23 as a partner of NRAMP1. The interaction was confirmed by split-mCitrine bimolecular fluorescence complementation and co-immunoprecipitation assays. In vitro phosphorylation assays pinpointed two CIPK23 target residues in NRAMP1, among which serine 20, important for endocytosis. Interestingly, Mn-induced internalization of NRAMP1 was unaffected by cipk23 mutation suggesting a potential redundancy between CIPK23 and other kinase(s). How CIPK23 could regulate NRAMP1 in response to Mn availability is discussed.
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