生物高聚物
自愈水凝胶
纳米技术
材料科学
化学
聚合物
高分子化学
复合材料
作者
Karen Xu,Nikolas Di Caprio,Hooman Fallahi,M. Dehghany,Matthew D. Davidson,Lorielle Laforest,Brian C.H. Cheung,Yuqi Zhang,Mingming Wu,Vivek B. Shenoy,Lin Han,Robert L. Mauck,Jason A. Burdick
标识
DOI:10.1038/s41467-024-46774-y
摘要
Abstract Cell migration is critical for tissue development and regeneration but requires extracellular environments that are conducive to motion. Cells may actively generate migratory routes in vivo by degrading or remodeling their environments or instead utilize existing extracellular matrix microstructures or microtracks as innate pathways for migration. While hydrogels in general are valuable tools for probing the extracellular regulators of 3-dimensional migration, few recapitulate these natural migration paths. Here, we develop a biopolymer-based bicontinuous hydrogel system that comprises a covalent hydrogel of enzymatically crosslinked gelatin and a physical hydrogel of guest and host moieties bonded to hyaluronic acid. Bicontinuous hydrogels form through controlled solution immiscibility, and their continuous subdomains and high micro-interfacial surface area enable rapid 3D migration, particularly when compared to homogeneous hydrogels. Migratory behavior is mesenchymal in nature and regulated by biochemical and biophysical signals from the hydrogel, which is shown across various cell types and physiologically relevant contexts (e.g., cell spheroids, ex vivo tissues, in vivo tissues). Our findings introduce a design that leverages important local interfaces to guide rapid cell migration.
科研通智能强力驱动
Strongly Powered by AbleSci AI