CD8型
启动(农业)
免疫系统
免疫学
黑色素瘤
免疫疗法
肿瘤微环境
细胞毒性T细胞
癌症研究
T细胞
癌症免疫疗法
生物
外周血单个核细胞
髓样
医学
生物化学
植物
发芽
体外
作者
Ramona Schlenker,Petra Schwalie,Steffen Dettling,Tamara Huesser,Anja Irmisch,Marisa Mariani,Julia M. Martínez Gómez,Alison Ribeiro,Florian Limani,Sylvia Herter,Emilio Yángüez,Sabine Hoves,Jitka Somandin,Juliane Siebourg‐Polster,Tony Kam‐Thong,Inês Matos,Pablo Umaña,Reinhard Dummer,Mitchell P. Levesque,Marina Bacac
出处
期刊:Med
[Elsevier]
日期:2024-04-01
标识
DOI:10.1016/j.medj.2024.03.015
摘要
The treatment of melanoma, the deadliest form of skin cancer, has greatly benefited from immunotherapy. However, many patients do not show a durable response, which is only partially explained by known resistance mechanisms.We performed single-cell RNA sequencing of tumor immune infiltrates and matched peripheral blood mononuclear cells of 22 checkpoint inhibitor (CPI)-naive stage III-IV metastatic melanoma patients. After sample collection, the same patients received CPI treatment, and their response was assessed.CPI responders showed high levels of classical monocytes in peripheral blood, which preferentially transitioned toward CXCL9-expressing macrophages in tumors. Trajectories of tumor-infiltrating CD8+ T cells diverged at the level of effector memory/stem-like T cells, with non-responder cells progressing into a state characterized by cellular stress and apoptosis-related gene expression. Consistently, predicted non-responder-enriched myeloid-T/natural killer cell interactions were primarily immunosuppressive, while responder-enriched interactions were supportive of T cell priming and effector function.Our study illustrates that the tumor immune microenvironment prior to CPI treatment can be indicative of response. In perspective, modulating the myeloid and/or effector cell compartment by altering the described cell interactions and transitions could improve immunotherapy response.This research was funded by Roche Pharma Research and Early Development.
科研通智能强力驱动
Strongly Powered by AbleSci AI