堆肥
放线菌门
微生物菌剂
厚壁菌
生物
食品科学
梭菌
微生物学
固氮
拟杆菌
肥料
微生物种群生物学
接种
细菌
16S核糖体RNA
园艺
农学
遗传学
作者
Rui Cao,Yihao Huang,Ruyu Li,Ke Li,Zhuqing Ren,Wu Jian
出处
期刊:PubMed
日期:2024-04-01
卷期号:16 (2): e13256-e13256
标识
DOI:10.1111/1758-2229.13256
摘要
This study examined the effects of three Bacillus strains and one Saccharomyces cerevisiae strain on nitrogen transformation and microbial communities in pig and chicken manure compost. The findings revealed that the use of compound microbial inoculants increased the compost temperature, accelerated moisture reduction, enhanced cellulase activity, and stimulated the accumulation of NH4+-N, NO3--N, and total nitrogen (TN), resulting in a 9% increase in TN content. The abundance of Firmicutes decreased by 3.95% at the maturation phase, while Actinobacteria and Bacteroidetes increased by 1.64% and 1.85%, respectively. Inoculation led to an increase in amoA, nxrA and nifH gene copy numbers, while simultaneously reducing the abundance of nirK, nosZ and nirS genes. It also resulted in an increase in functional enzyme levels, specifically nif and amo, with a corresponding decrease in nor. Clostridium, Phascolarctobacterium, Eubacterium and Faecalibacterium from the class Clostridium, which have a significant correlation with nifH and nxrA genes, suggest their likely crucial role in nitrogen retention and fixation. Inoculation aided in the removal of pathogenic bacteria and antibiotic resistance genes (ARGs) like fluoroquinolones, nucleosides and nitroimidazole. This study provides effective theoretical support for the mechanism of nitrogen retention and fixation, and for improving the quality of compost.
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