Multilayer Electrospun-Aligned Fibroin/Gelatin Implant for Annulus Fibrosus Repair: An In Vitro and In Vivo Evaluation

丝素 明胶 生物医学工程 椎间盘 椎间盘切除术 脚手架 材料科学 植入 体内 静电纺丝 生物相容性 组织工程 极限抗拉强度 纳米纤维 成纤维细胞 解剖 化学 丝绸 体外 聚合物 纳米技术 医学 复合材料 腰椎 外科 生物 生物化学 冶金 生物技术
作者
Ming‐Hsiao Hu,Kai‐Chiang Yang,Chih‐Wei Chen,Po‐Han Chu,Yun-Liang Chang,Yuan‐Hui Sun,Feng‐Huei Lin,Shuhua Yang
出处
期刊:Biomedicines [Multidisciplinary Digital Publishing Institute]
卷期号:10 (9): 2107-2107 被引量:4
标识
DOI:10.3390/biomedicines10092107
摘要

Annulus fibrosus (AF) damage is proven to prompt intervertebral disc (IVD) degeneration, and unrepaired AF lesions after surgical discectomy may boost herniation of the nucleus pulposus (NP) which may lead to further compression of neural structures. Moreover, vascular and neural ingrowth may occur within the defect which is known as a possible reason for discogenic pain. Due to a limited healing capacity, an effective strategy to repair and close the AF defect is necessary. In this study, using electrospinning technology, two nature polymers, silk fibroin and gelatin, were linked to imitate the unique lamellae structure of native AF. Our findings revealed that a multilayer electrospun-aligned fibroin/gelatin scaffold with mechanical and morphological properties mimicking those of native AF lamellae have been developed. The average diameter of the nanofiber is 162.9 ± 38.8 nm. The young’s modulus is around 6.70 MPa with an ultimate tensile strength of around 1.81 MP along preferred orientation. The in vitro test confirmed its biocompatibility and ability to maintain cell viability and colonization. Using a porcine model, we demonstrated that the multilayer-aligned scaffold offered a crucial microenvironment to induce collagen fibrous tissue production within native AF defect. In the implant-repaired AF, H&E staining showed homogeneous fibroblast-like cell infiltration at the repaired defect with very little vascular ingrowth, which was confirmed by magnetic resonance imaging findings. Picrosirius red staining and immunohistochemical staining against type I collagen revealed positively stained fibrous tissue in an aligned pattern within the implant-integrated site. Relative to the intact control group, the disc height index of the serial X-ray decreased significantly in both the injury control and implant group at 4 weeks and 8 weeks (p < 0.05) which indicated this scaffold may not reverse the degenerative process. However, the results of the discography showed that the effectiveness of annulus repair of the implant group is much superior to that of the untreated group. The scaffold, composed with nature fibroin/gelatin polymers, could potentially enhance AF healing that could prevent IVD recurrent herniation, as well as neural and neovascular ingrowth after discectomy surgeries.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
lee完成签到,获得积分10
1秒前
1秒前
lalala发布了新的文献求助10
3秒前
陶醉的熊完成签到,获得积分10
3秒前
3秒前
3秒前
3秒前
贺丞完成签到,获得积分10
4秒前
4秒前
酷酷涫完成签到 ,获得积分0
4秒前
5秒前
量子星尘发布了新的文献求助10
5秒前
折镜发布了新的文献求助10
8秒前
why发布了新的文献求助10
8秒前
9秒前
10秒前
11秒前
折镜完成签到,获得积分10
13秒前
wmufwd完成签到,获得积分10
13秒前
13秒前
zhw发布了新的文献求助10
14秒前
8R60d8应助欢城采纳,获得10
14秒前
天子骄子发布了新的文献求助10
14秒前
Mida发布了新的文献求助10
15秒前
高贵紫丝完成签到,获得积分10
15秒前
szc完成签到,获得积分10
15秒前
林子发布了新的文献求助10
16秒前
17秒前
Lucas应助JUGG采纳,获得10
18秒前
18秒前
T_MC郭发布了新的文献求助10
19秒前
19秒前
19秒前
why完成签到,获得积分10
20秒前
20秒前
汉堡包应助witting采纳,获得10
20秒前
qiang发布了新的文献求助10
20秒前
21秒前
22秒前
23秒前
高分求助中
【提示信息,请勿应助】请使用合适的网盘上传文件 10000
Continuum Thermodynamics and Material Modelling 2000
Chinesen in Europa – Europäer in China: Journalisten, Spione, Studenten 1200
Deutsche in China 1920-1950 1200
Electron microscopy study of magnesium hydride (MgH2) for Hydrogen Storage 800
Green Star Japan: Esperanto and the International Language Question, 1880–1945 800
Sentimental Republic: Chinese Intellectuals and the Maoist Past 800
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3871187
求助须知:如何正确求助?哪些是违规求助? 3413299
关于积分的说明 10683969
捐赠科研通 3137766
什么是DOI,文献DOI怎么找? 1731163
邀请新用户注册赠送积分活动 834643
科研通“疑难数据库(出版商)”最低求助积分说明 781250