葡萄糖氧化酶
壳聚糖
化学
抗菌活性
淀粉
明胶
核化学
大肠杆菌
高分子化学
细菌
生物化学
组合化学
酶
生物
基因
遗传学
作者
Yufei Cao,Juanjuan Yin,Yuting Shi,Cheng Ju,F. Richard Yu,Congshu Huang,Wenbin Yu,Mingsheng Liu,Zheng Yang,Haicun Zhou,Hongbin Liu,Jianrong Wang,Guanghui Zhao
标识
DOI:10.1016/j.ijbiomac.2022.08.152
摘要
In this work, a positively charged chitosan-grafted-polyarginine (CS-N-PArg) as the macro-molecular NO donor, and a negatively charged acetalated starch (AcSt-O-PAsp) as a glucose donor, have been synthesized. To achieve the multi-enzymatic cascade system for local generation of self-supply glucose to increase the H2O2 concentration for the subsequent oxidization of L-Arg into NO, the designed positively charged CS-N-PArg, negatively charged AcSt-O-PAsp, glucoamylase (GA) and glucose oxidase (GOx) are absorbed and assembled in the pore of the gelatin sponge via electrostatic interaction to establish a smart antibacterial dressings (CS/St + GOx/GA). Once stimulated by Escherichia coli (E. coli)-infected wounds (a slightly acidic environment), the cascade reaction system can sequentially induce to generate glucose, H2O2 and NO, which exhibits a meaningful alternative idea for a high-performance antibacterial therapy.
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