小泡
膜
鞘磷脂
细胞器
化学
细胞质
细胞生物学
细胞
生物化学
细胞膜
生物物理学
生物
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1976-11-12
卷期号:194 (4266): 743-745
被引量:252
标识
DOI:10.1126/science.982044
摘要
Monolayer cell cultures of macrophages, monocytes, myoblasts, and density-inhibited and transformed fibroblasts form and release cell surface membrane vesicles following exposure to formaldehyde, related low-molecular-weight aldehydes, and disulfide blocking agents. Vesicles have a unique composition of proteins and lipids. They show enrichment of cholesterol and sphingomyelin content and a seven-to tenfold enrichment of 5'-nucleotidase activity. Vesicles also contain intramembranous particles and show a trilamellar unit membrane and no ultrastructural evidence of contamination with other cytoplasmic organelles. The technique is proposed as a novel method for isolating plasma membrane vesicles from cells in culture.
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