生物
多路复用
多路复用
寡核苷酸
染色质
计算生物学
DNA
计算机科学
生物信息学
遗传学
电信
作者
Kaile Wang,Zhenna Xiao,Yun Yan,Rui Ye,Min Hu,Shanshan Bai,Emi Sei,Yawei Qiao,Hui Chen,Bora Lim,Steven H. Lin,Nicholas E. Navin
出处
期刊:Molecular Cell
[Elsevier]
日期:2021-10-01
卷期号:81 (20): 4319-4332.e10
被引量:18
标识
DOI:10.1016/j.molcel.2021.09.026
摘要
Microdroplet single-cell ATAC-seq is widely used to measure chromatin accessibility, however, highly scalable and simple sample multiplexing procedures are not available. Here, we present a transposome-assisted single nucleus barcoding approach for ATAC-seq (SNuBar-ATAC) that utilizes a single oligonucleotide adaptor for multiplexing samples during the existing tagmentation step and does not require a pre-labeling procedure. The accuracy and scalability of SNuBar-ATAC was evaluated using cell line mixture experiments. We applied SNuBar-ATAC to investigate treatment-induced chromatin accessibility dynamics by multiplexing 28 mice with lung tumors that received different combinations of chemo, radiation, and targeted immunotherapy. We also applied SNuBar-ATAC to study spatial epigenetic heterogeneity by multiplexing 32 regions from a human breast tissue. Additionally, we show that SNuBar can multiplex single cell ATAC and RNA multiomic assays in cell lines and human breast tissue samples. Our data show that SNuBar is a highly accurate, easy-to-use, and scalable system for multiplexing scATAC-seq and scATAC and RNA co-assay experiments.
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