材料科学
静电纺丝
细胞外基质
纳米纤维
聚己内酯
生物材料
细胞迁移
细胞粘附
组织工程
粘附
生物物理学
聚酯纤维
纳米技术
纳米地形
微观结构
细胞生物学
细胞
化学
生物医学工程
复合材料
聚合物
医学
生物化学
生物
作者
Yang Zhang,Xiaofeng Wang,Yan Zhang,Yajing Liu,Dongfang Wang,Xueke Yu,Haonan Wang,Zhiyuan Bai,Yu Jiang,Yongming Li,Wei Zheng,Qian Li
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2021-09-20
卷期号:7 (10): 4959-4970
被引量:12
标识
DOI:10.1021/acsbiomaterials.1c00951
摘要
The study of cell migration on biomaterials is of great significance in tissue engineering and regenerative medicine. In recent years, there has been increasing evidence that the physical properties of the extracellular matrix (ECM), such as surface topography, affect various cellular behaviors such as proliferation, adhesion, and migration. However, the biological mechanism of surface topography influencing cellular behavior is still unclear. In this study, we prepared polycaprolactone (PCL) fibrous materials with different surface microstructures by solvent casting, electrospinning, and self-induced crystallization. The corresponding topographical structure obtained is a two-dimensional (2D) flat surface, 2.5-dimensional (2.5D) fibers, and three-dimensional (3D) fibers with a multilevel microstructure. We then investigated the effects of the complex topographical structure on endothelial cell migration. Our study demonstrates that cells can sense the changes of micro- and nanomorphology on the surface of materials, adapt to the physical environment through biochemical reactions, and regulate actin polymerization and directional migration through Rac1 and Cdc42. The cells on the nanofibers are elongated spindles, and the positive feedback of cell adhesion and actin polymerization along the fiber direction makes the plasma membrane continue to protrude, promoting cell polarization and directional migration. This study might provide new insights into the biomaterial design, especially those used for artificial vascular grafts.
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