新生霉素
羧苄青霉素
ATP结合盒运输机
流出
铜绿假单胞菌
微生物学
生物
多重耐药
维拉帕米
化学
生物化学
抗生素
运输机
细菌
氨苄西林
基因
遗传学
有机化学
钙
作者
Christian Hulen,Pierre‐Jean Racine,Marc Feuilloley,Abdelhakim Elomri,Noureddine Lomri
出处
期刊:Antibiotics
[MDPI AG]
日期:2022-05-21
卷期号:11 (5): 700-700
被引量:5
标识
DOI:10.3390/antibiotics11050700
摘要
The biological effects of alkaloids, curine, guattegaumerine, and verapamil, on Pseudomonas aeruginosa were investigated. These molecules did not inhibit P. aeruginosa growth but increased the sensitivity of this bacterium to carbenicillin, novobiocin, and erythromycin. The results of another study indicate that curine and guattegaumerine were competitors of verapamil and acted as inhibitors of eukaryotic ABCB1 efflux pump. A BLAST-P carried out between a bacterial MDR transporter LmrA from Lactococcus lactis, a human MDR1/P-glycoprotein (ABCB1), and ABC proteins of P.aeruginosa highlighted five potential candidates that have this bacterium. A study on the sensitivity to carbenicillin in the presence of verapamil allowed us to identify the product of gene PA1113 as the ABC transporter involved in the influx of carbenicillin. Similarly, novobiocin transport performed in the presence of verapamil and a docking analysis highlighted protein MsbA (Lipid A flippase, gene PA4997) as a potential candidate in novobiocin efflux. MsbA has previously been identified as a multidrug transporter in E. coli, and as P. aeruginosa MsbA presented 76% identity with E. coli MsbA, it is possible that novobiocin efflux involves this ABC transporter, accounting for about 30% of the bacterium resistance to this antibiotic.
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