葡萄糖氧化酶
聚丙烯腈
检出限
生物传感器
静电纺丝
固定化酶
选择性
Nafion公司
化学
基质(化学分析)
纳米颗粒
涂层
材料科学
化学工程
色谱法
酶
纳米技术
催化作用
生物化学
聚合物
有机化学
电化学
电极
物理化学
工程类
作者
Roxana‐Mihaela Apetrei,Pınar Çamurlu
标识
DOI:10.1016/j.bioelechem.2021.107751
摘要
The current study aims to provide a valid comparison between glucose detection efficiency with an enzymatic and a non-enzymatic sensing platform. A low-cost nano-matrix for glucose sensing was developed by drop-coating copper nanoparticles (Cu NPs) onto a polyacrylonitrile (PAN) electrospun nanofibrous assembly. The PAN NFs/Cu NPs matrix was optimized regarding electrospinning time and Cu NPs content and employed as a non-enzymatic sensor or further modified by cross-linking of glucose oxidase (GOD) for the development of an enzymatic sensor. The non-enzymatic glucose sensor was three times more sensitive (300 mAM−1cm−2) than the enzymatic one (81 mAM−1cm−2) with similar limit of detection values (5.9 and 5.6 µM, respectively). Incorporation of MWCNTs improved both the LOD (3.3 µM) and the operational stability of the non-enzymatic configuration (RSD 7.3%). The interference effect proved insignificant for the enzymatic sensor due to the innate catalytic selectivity whilst the non-enzymatic sensor acquired selectivity due to the nanofibrous PAN matrix and Nafion coating. The non-enzymatic PAN NFs/Cu NPs sensor was chosen for the detection of glucose in real blood serum samples whilst the PAN NFs/Cu NPs/GOD sensor was applied for glucose detection in fruit juices, both proving recovery results close to 100%.
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