纳米传感器
费斯特共振能量转移
体内
生物物理学
荧光
化学
纳米技术
材料科学
生物
量子力学
物理
生物技术
作者
Peng Yu,Kui Yan,Shangfeng Wang,Chenzhi Yao,Zuhai Lei,Yaohui Tang,Fan Zhang
出处
期刊:Nano Letters
[American Chemical Society]
日期:2022-12-01
卷期号:22 (23): 9732-9740
被引量:7
标识
DOI:10.1021/acs.nanolett.2c04084
摘要
Ratiometric fluorescence nanosensors provide quantitative biological information. However, spectral shift and distortion of ratiometric nanosensors in biological media often compromise sensing accuracy, limiting in vivo applications. Here, we develop a fluorescent dyad (aBOP-IR1110) in the second near-infrared (NIR-II) window by covalently linking an asymmetric aza-BODIPY with a ONOO--responsive meso-thiocyanine. The dyad encapsulated in the PEGylated nanomicelle largely improves spectral fidelity in serum culture by >9.4 times compared to that of its noncovalent counterpart. The increased molecular weights (>1480 Da) and hydrophobicity (LogP of 7.87-12.36) lock dyads inside the micelles, which act as the shield against the external environment. ONOO--altered intramolecular Förster resonance energy transfer (FRET) generates linear ratiometric response with better serum tolerance, enabling us to monitor the dynamics of oxidative stress in traumatic brain injury and evaluate therapeutic efficiency. The results show high correlation with in vitro triphenyltetrazolium chloride staining, suggesting the potential of NIR-II dyad-doped nanosensor for in vivo high-fidelity sensing applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI