糖酵解
癌症研究
厌氧糖酵解
间充质干细胞
纤维化
基因敲除
化学
细胞生物学
医学
内科学
生物
生物化学
新陈代谢
细胞凋亡
作者
Shihao Xu,Yin Celeste Cheuk,Yichen Jia,Tian Chen,Juntao Chen,Yongsheng Luo,Yirui Cao,Jingjing Guo,Lijun Dong,Yi Zhang,Yi Shi,Ruiming Rong
标识
DOI:10.1038/s41419-022-05305-7
摘要
Renal fibrosis is a common pathological feature and outcome of almost all chronic kidney diseases, and it is characterized by metabolic reprogramming toward aerobic glycolysis. Mesenchymal stem cell-derived exosomes (MSC-Exos) have been proposed as a promising therapeutic approach for renal fibrosis. In this study, we investigated the effect of MSC-Exos on glycolysis and the underlying mechanisms. We demonstrated that MSC-Exos significantly ameliorated unilateral ureter obstruction (UUO)-induced renal fibrosis by inhibiting glycolysis in tubular epithelial cells (TECs). miRNA sequencing showed that miR-21a-5p was highly enriched in MSC-Exos. Mechanistically, miR-21a-5p repressed the expression of phosphofructokinase muscle isoform (PFKM), a rate-limiting enzyme of glycolysis, thereby attenuating glycolysis in TECs. Additionally, knockdown of miR-21a-5p abolished the renoprotective effect of MSC-Exos. These findings revealed a novel role for MSC-Exos in the suppression of glycolysis, providing a new insight into the treatment of renal fibrosis.
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