生物
嵌合体(遗传学)
互补
经济短缺
肺
胚泡
细胞生物学
移植
免疫学
胚胎
表型
遗传学
胚胎发生
基因
内科学
医学
语言学
哲学
政府(语言学)
作者
Shunsuke Yuri,Yuki Murase,Ayako Isotani
出处
期刊:Development
[The Company of Biologists]
日期:2024-01-01
卷期号:151 (1)
被引量:1
摘要
ABSTRACT Regenerative medicine is a tool to compensate for the shortage of lungs for transplantation, but it remains difficult to construct a lung in vitro due to the complex three-dimensional structures and multiple cell types required. A blastocyst complementation method using interspecies chimeric animals has been attracting attention as a way to create complex organs in animals, although successful lung formation using interspecies chimeric animals has not yet been achieved. Here, we applied a reverse-blastocyst complementation method to clarify the conditions required to form lungs in an Fgfr2b-deficient mouse model. We then successfully formed a rat-derived lung in the mouse model by applying a tetraploid-based organ-complementation method. Importantly, rat lung epithelial cells retained their developmental timing even in the mouse body. These findings provide useful insights to overcome the barrier of species-specific developmental timing to generate functional lungs in interspecies chimeras.
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