壳聚糖
抗菌活性
化学
最小抑制浓度
银纳米粒子
溶血
核化学
细胞毒性
大肠杆菌
最低杀菌浓度
细菌细胞结构
微生物学
纳米颗粒
生物物理学
细菌
纳米技术
生物化学
体外
材料科学
生物
基因
遗传学
免疫学
作者
Sovan Samanta,Jhimli Banerjee,Balaram Das,Jayanta Mandal,Soumendranath Chatterjee,Kazi Monjur Ali,Sangram Sinha,Biplab Giri,Totan Ghosh,Sandeep Kumar Dash
标识
DOI:10.1016/j.ijbiomac.2022.08.050
摘要
In the study, leaf extract of Carica papaya was utilized for the biogenic fabrication process of chitosan functionalized silver nanoparticles (Ag-Chito NPs). HRTEM analysis revealed that the fabricated Ag-Chito NPs was spherical in shape, with an average particle size of 13.31 (±0.07) nm. FTIR, UV-Vis, DLS, and other characterizations were also performed to analyze the diverse physicochemical properties of the particles. The antibacterial potency of the synthesized Ag-Chito NPs was tested against the two clinically isolated multidrug resistant uropathogenic bacterial strains, i.e. MLD 2 (Escherichia coli) and MLD 4 (Staphylococcus aureus) through MIC, MBC, time and concentration dependent killing kinetic assay, inhibition of biofilm formation assay, fluorescence and SEM imaging. Significantly, Ag-Chito NPs showed the highest sensitivity against the MLD 2 (MIC value of 12.5 μg/mL) strain, as compared to the MLD 4 (MIC value of 15 μg/mL) strain. From the hemolysis assay, it was revealed that Ag-Chito NPs exerted no significant toxicity up to 50 μg/mL against healthy human blood cells. Additionally, in silico analysis of chitosan (functionalized on the surface of AgNPs) and bacterial cell membrane protein also evidently suggested a strong interaction between Ag-Chito NPs and bacterial cells, which might be responsible for bacterial cell death.
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