荧光
材料科学
吸附
级联
氧气
电子转移
化学工程
催化作用
光化学
纳米技术
组合化学
化学
光学
物理化学
物理
工程类
色谱法
有机化学
作者
Zhibo Tong,Tong Wang,Yunheng Cai,Jingquan Sha,Tai Peng
摘要
Enzyme cascade reactions in organisms have sparked tremendous interest for their coupled catalysis-facilitated efficient biochemical reactions. However, multi-enzyme cascade nanozymes remain largely unused. In the work, flower-like porous ceria-based integrated enzymes (INAzyme), FeMo6@CeO2 (FMC-n), were readily prepared using an efficient thermally induced process. Owing to a larger specific surface area and excellent adsorption, the flower-like matrix (CeO2) can serve as not only an effective sorbent for the conversion of dissolved oxygen in solution but also an excellent support for self-cascade reactions with FeMo6. Based on the electron transfer through Fe and Ce cycles, FMC-n INAzyme exhibits intrinsic oxidase-, peroxidase- and Fenton-like activities. Moreover, by assessing the Vo mobility and F-Vo relative content of FMC-2 and FC-66, we found that the contribution of enhanced accessibility through the specific surface area (SSA) to the activity of the INAzyme is significantly higher than the active sites. Finally, a fluorescence turn-on enhancement sensing platform based on self-cascade FMC-n for the detection of Cys was established, culminating in a LOD of as low as 0.014 μM in the range of 1-100 μM.
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