川地31
肝细胞癌
裸鼠
移植
免疫组织化学
病理
川地34
癌症研究
血管生成
医学
肝肿瘤
肝移植
肝母细胞瘤
癌
组织学
生物
癌症
干细胞
放射科
内科学
遗传学
作者
Zhiting Xu,Hong Ding,Tiantian Fu,Yu-li Zhu,Wenping Wang
出处
期刊:Medical Science Monitor
[International Scientific Information, Inc.]
日期:2019-11-18
卷期号:25: 8694-8703
被引量:3
摘要
BACKGROUND:This study aimed to develop a nude mouse model of orthotopic liver transplantation of HCCLM3 human hepatocellular carcinoma (HCC) cell xenografts and the use of imaging and histology to evaluate tumor development and progression. MATERIAL AND METHODS:HCCLM3 cells were injected subcutaneously into 25 healthy male athymic BALB/c (nu/nu) nude mice. The tumors that developed were transplanted into the liver of a new set of nude mice. After four weeks and six weeks, the mice were imaged using ultrasound (US), software-assisted contrast-enhanced ultrasound (CEUS), fluorodeoxyglucose-positron emission tomography (FDG-PET). Histology was performed on the liver and liver tumors, and included immunohistochemistry for vascular endothelial growth factor (VEGF), CD31, CD34, and α-smooth muscle actin (α-SMA). RESULTS:The success rate for orthotopic tumor transplantation in the mouse liver was 90% (18/20). Liver tumors measured 11.8±2.6 mm in diameter and 525.9±250.8 mm3 in volume on the sixth week. CEUS showed rapid wash-in and washout in the liver tumors, and PET showed low tumor cell metabolism. Bone metastases were present in 45% (9/20) of mice in the sixth week. Immunohistochemistry showed positive expression for VEGF, CD31, CD34, and α-SMA. CONCLUSIONS:The nude mouse orthotopic liver transplantation model of human HCC was shown to be a reliable model that has the potential for future research on the pathogenesis and progression of HCC and studies on drug development.
科研通智能强力驱动
Strongly Powered by AbleSci AI