心肌细胞
细胞生物学
骨骼肌
基底层
离体
再生(生物学)
生物
染色
肌发生
人口
卫星
解剖
病理
体内
超微结构
医学
遗传学
工程类
航空航天工程
环境卫生
作者
Yann S. Gallot,Sajedah M. Hindi,Aman K Mann,Ashok Kumar
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2016-01-01
卷期号:6 (19)
被引量:17
标识
DOI:10.21769/bioprotoc.1942
摘要
Adult skeletal muscle regeneration is orchestrated by a specialized population of adult stem cells called satellite cells, which are localized between the basal lamina and the plasma membrane of myofibers. The process of satellite cell-activation, proliferation, and subsequent differentiation that occurs during muscle regeneration can be recapitulated ex vivo by isolation of single myofibers from skeletal muscles and culturing them under suspension conditions. Here, we describe an improved protocol to evaluate ex vivo satellite cells activation through isolation of single myofibers from extensor digitorum longus (EDL) muscle of mice and culturing and staining of myofiber-associated satellite cells with the markers of self-renewal, proliferation, and differentiation.
科研通智能强力驱动
Strongly Powered by AbleSci AI