Coculture of a Pathogenic Actinomycete and Animal Cells To Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect

A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554T and the mouse macrophage-like cell line J774.1 in a modified Czapek–Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.