DNA甲基化
精子
甲基化
男科
染色质
精液
DNA
生物
男性不育
生育率
精子活力
精子质量
精液分析
分子生物学
遗传学
医学
不育
怀孕
基因
基因表达
人口
环境卫生
作者
Mohammed Hamad,Wael Abu Dayyih,Mohammad Laqqan,Y. Alkhaled,Mathias Montenarh,Mohamad Eid Hammadeh
标识
DOI:10.1016/j.rbmo.2018.08.016
摘要
Research question Does regular smoking affect semen quality and the levels of DNA methylation in mature human spermatozoa? Design Spermatozoa from 109 men were evaluated (55 smokers and 54 non-smokers). DNA was extracted from purified spermatozoa, and DNA methylation was quantified by enzyme-linked immunosorbent assay (ELISA). Results Global DNA methylation of non-smokers is significantly lower (P < 0.001) than that of smokers (4.85 ± 2.72 and 7.08 ± 1.77 ng/μl, respectively). Moreover, the mean global DNA methylation levels were significantly correlated (r = 0.22;P = 0.02) with non-condensed chromatin in the spermatozoa. Levels of non-condensed chromatin were significantly higher (P < 0.001) in smokers (29.75 ± 9.38%) compared with non-smokers (20.96 ± 11.31%). Furthermore, global sperm DNA methylation was negatively correlated with high significance (P < 0.010) with sperm: count (r = –0.27), motility (r = –0.30) and vitality (r = –0.26). Conclusion Smoking interferes with DNA methylation. Also, DNA methylation is significantly correlated with sperm parameters and sperm non-condensed chromatin. These data emphasize another detrimental effect of smoking on male fertility. DNA methylation may, therefore, be considered as a fertility marker in men.
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