Emulsion Template Method for the Fabrication of Gelatin-Based Scaffold with a Controllable Pore Structure

The porous microstructure of scaffolds is an essential consideration for tissue engineering, which plays an important role for cell adhesion, migration, and proliferation. It is crucial to choose optimum pore sizes of scaffolds for the treatment of various damaged tissues. Therefore, the proper porosity is the significant factor that should be considered when designing tissue scaffolds. Herein, we develop an improved emulsion template method to fabricate gelatin-based scaffolds with controllable pore structure. Gelatin droplets were first prepared by emulsification and then solidified by genipin to prepare gelatin microspheres. The microspheres were used as a template for the fabrication of porous scaffolds, which were gathered and tightened together by dialdehyde amylose. The results showed that emulsification can produce gelatin microspheres with narrow size distribution. The size of gelatin microspheres was easily controlled by adjusting the concentration of gelatin and the speed of mechanical agitation. The gelatin-based scaffolds presented macroporous and interconnected structure. It is interesting that the pore size of scaffolds was directly related to the size of gelatin microspheres, displaying the same trend of change in size. It indicated that the gelatin microspheres can be used as the proper template to fabricate gelatin-based scaffold with a desired pore structure. In addition, the gelatin-based scaffolds possessed good blood compatibility and cytocompatibility. Overall, the gelatin-based scaffolds exhibited great potential in tissue engineering.