生物相容性
生物信息学
体外
化学
核化学
药理学
生物化学
有机化学
生物
基因
作者
Vladimir V. Sharoyko,Nailia R. Iamalova,Sergei V. Ageev,Anatolii A. Meshcheriakov,Gleb O. Iurev,Andrey V. Petrov,Dmitry Nerukh,Vladimir Farafonov,Lubov V. Vasina,Anastasia Penkova,Konstantin N. Semenov
标识
DOI:10.1021/acs.jpcb.1c03332
摘要
Light fullerenes, C60 and C70, have significant potential in biomedical applications due to their ability to absorb reactive oxygen species, inhibit the development of tumors, inactivate viruses and bacteria, and as the basis for developing systems for targeted drug delivery. However, the hydrophobicity of individual fullerenes complicates their practical use; therefore, creating water-soluble derivatives of fullerenes is increasingly important. Currently, the most studied soluble adducts of fullerenes are polyhydroxy fullerenes or fullerenols. Unfortunately, investigations of fullerenol biocompatibility are fragmental. They often lack reproducibility both in the synthesis of the compounds and their biological action. We here investigate the biocompatibility of a well-defined fullerenol C60(OH)24 obtained using methods that minimize the content of impurities and quantitatively characterize the product's composition. We carry out comprehensive biochemical and biophysical investigations of C60(OH)24 that include photodynamic properties, cyto- and genotoxicity, hemocompatibility (spontaneous and photo-induced hemolysis, platelet aggregation), and the thermodynamic characteristics of C60(OH)24 binding to human serum albumin and DNA. The performed studies show good biocompatibility of fullerenol C60(OH)24, which makes it a promising object for potential use in biomedicine.
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