材料科学
检测点注意事项
自愈水凝胶
注意事项
分析物
纳米技术
仪表(计算机编程)
生物医学工程
软件可移植性
计算机科学
色谱法
化学
工程类
操作系统
生物
护理部
医学
高分子化学
程序设计语言
免疫学
作者
Dan Liu,Shasha Jia,Huimin Zhang,Yanli Ma,Zhichao Guan,Jiuxing Li,Zhi Zhu,Tao Ji,Chaoyong Yang
标识
DOI:10.1021/acsami.7b05531
摘要
Point-of-care testing (POCT) with the advantages of speed, simplicity, and low cost, as well as no need for instrumentation, is critical for the measurement of analytes in a variety of environments lacking access to laboratory infrastructure. In the present study, a hydrogel pressure-based assay for quantitative POCT was developed by integrating a target-responsive hydrogel with pressuremeter readout. The target-responsive hydrogels were constructed with DNA grafted linear polyacrylamide and the cross-linking DNA for selective target recognition. The hydrogel response to the target substance allows release of the preloaded Pt nanoparticles, which have good stability and excellent catalytic ability for decomposing H2O2 to O2. Then, the generated O2 in a sealed environment leads to significant pressure increase, which can be easily read out by a handheld pressuremeter. Using this target-responsive hydrogel pressure-based assay, portable and highly sensitive detection of cocaine, ochratoxin A, and lead ion were achieved with excellent accuracy and selectivity. With the advantages of portability, high sensitivity, and simple sample processing, the hydrogel pressure-based assay shows great potential for quantitative POCT of a broad range of targets in resource-limited settings.
科研通智能强力驱动
Strongly Powered by AbleSci AI