内吞作用
材料科学
胞吐
纳米颗粒
纳米技术
赫拉
生物物理学
内吞循环
细胞内
荧光
化学
膜
细胞
生物化学
生物
光学
物理
作者
Yun Mi Bae,Yong Il Park,Sang Hwan Nam,Jeong Hyun Kim,Kyung‐Hee Lee,Hyung Min Kim,Byeongjun Yoo,Joon Sig Choi,Jun Young Lee,Taeghwan Hyeon,Yung Doug Suh
出处
期刊:Biomaterials
[Elsevier]
日期:2012-12-01
卷期号:33 (35): 9080-9086
被引量:103
标识
DOI:10.1016/j.biomaterials.2012.08.039
摘要
Lanthanide-doped upconverting nanoparticles (UCNPs) have recently attracted enormous attention in the field of biological imaging owing to their unique optical properties (near-infrared excitation followed by photoluminescence in the visible spectral range). For biological applications, it is critical to understand the interaction between these nanoparticles and biological systems at the cellular level. In this paper, using epi-fluorescence microscopy with 980-nm excitation, a full intracellular pathway composed of endocytosis, active transport, and exocytosis was clearly visualized for PEG-phospholipid-coated UCNPs in single HeLa cells, which was experimentally feasible mostly thanks to the excellent photostability and low cytotoxicity thereof. Each step in the pathway was characterized and identified by various chemical inhibition studies and spectroscopic measurements.
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