戊二醛
纤维素
共价键
化学
高分子化学
环氧树脂
刀豆蛋白A
基质(化学分析)
配体(生物化学)
化学工程
有机化学
色谱法
生物化学
工程类
受体
体外
作者
Jolita Aniulyte,Jolanta Bryjak,Jolanta Liesienė
出处
期刊:Proceedings of the Estonian Academy of Sciences
日期:2006-01-01
卷期号:55 (2): 61-61
被引量:4
标识
DOI:10.3176/chem.2006.2.02
摘要
A method for the activation of a cellulose-based matrix resulting in obtaining active primary amino groups via a long spacer arm was developed. The method is based on coupling pentaethylenehexamine (PEHA) to the polymeric matrix through epoxy groups. It was shown that modification of cellulose with PEHA is a straightforward and convenient procedure that has the advantages of high coupling efficiency. The primary amino group density on the carrier surf ace can be easily adjusted by changing the content of epoxy groups in the matrix or by altering the amount of PEHA in the reaction mixture. The lectins Concanavalin A and Wheat Germ Agglutinin were employed as ligands for the immobilization onto the PEHA-activated carrier using glutaraldehyde. It was shown that the spacer arm affected ligand coupling kinetics as well as the chromatographic behaviour of the adsorbents obtained. Covalent immobilization of enzyme glucoamylase on PEHA-activated cellulose was done. It was found that covalent binding via glutaraldehyde offers satisfactorily stable and active preparations.
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