细胞周期
肿瘤坏死因子α
细胞生物学
生物
中间性中心性
微阵列分析技术
促炎细胞因子
细胞
细胞周期检查点
内皮干细胞
计算生物学
基因
免疫学
炎症
基因表达
遗传学
中心性
组合数学
体外
数学
作者
Yang Chen,Jin Gu,Dan Li,Shao Li
出处
期刊:Bioinformatics
[Oxford University Press]
日期:2011-11-15
卷期号:28 (1): 1-4
被引量:35
标识
DOI:10.1093/bioinformatics/btr619
摘要
Abstract Motivation: Tumor necrosis factor-alpha (TNF-α), a major inflammatory cytokine, is closely related to several cardiovascular pathological processes. However, its effects on the cell cycle of vascular endothelial cells (VECs) have been the subject of some controversy. To investigate the molecular mechanism underlying this process, we constructed time-course protein–protein interaction (PPI) networks of TNF-α induced regulation of cell cycle in VECs using microarray datasets and genome-wide PPI datasets. Then, we analyzed the topological properties of the responsive PPI networks and calculated the node degree and node betweenness centralization of each gene in the networks. We found that p21, p27 and cyclinD1, key genes of the G1/S checkpoint, are in the center of responsive PPI networks and their roles in PPI networks are significantly altered with induction of TNF-α. According to the following biological experiments, we proved that TNF-α can promote G1/S transition of cell cycle in VECs and facilitate the cell cycle activation induced by vascular endothelial growth factor. Contact: shaoli@mail.tsinghua.edu.cn Supplementary information: Supplementary data are available at Bioinformatics online.
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