人巨细胞病毒
巨细胞病毒
病毒学
抗体
免疫系统
生物
酶
细胞因子
抗原
免疫学
分子生物学
病毒
疱疹病毒科
病毒性疾病
生物化学
作者
Vivian P. Young,Margarette C. Mariano,Lionel Faure,Juliet V. Spencer
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 291-299
被引量:4
标识
DOI:10.1007/978-1-0716-1111-1_15
摘要
Since its introduction in 1971, the enzyme-linked immunosorbent assay (ELISA) has revolutionized medicine by enabling detection of both antigens and antibodies in a variety of samples. We describe here a customized sandwich ELISA developed for the detection of Human Cytomegalovirus interleukin-10 (cmvIL-10). CmvIL-10 is a virally encoded cytokine and ortholog of human interleukin 10 (hIL-10). While cmvIL-10 and hIL-10 are similar in structure and function, overall amino acid sequence identity is only 27%, resulting in antigenically distinct proteins. The cmvIL-10 ELISA is specific and does not detect hIL-10. The assay is sensitive enough to detect cmvIL-10 in both culture supernatants and patient serum. The ability to quantify cmvIL-10 levels during HCMV infection could provide valuable information about immune evasion strategies and viral control of host signaling pathways.
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